• Protein designing in silico and molecular cloning • Expression, purification and characterization of proteins • Development of LC-MS/MS based analytical methods • Proteomics/nutrigenomics study of seed proteins • Purification and characterization of pectic enzymes
Biotechnology, Biochemistry, Analytical Chemistry, Foodomics
1. Sunil L and Prasanna Vasu. In silico Designing of Therapeutic Protein enriched with Branched-Chain Amino Acids for the Dietary Treatment of Chronic Liver Disease. Journal of Molecular Graphics and Modelling (In Press). 2. Brett J. Savary, Prasanna Vasu, Randall G. Cameron, T. Gregory McCollum, and Alberto Nuñez. 2013. Structural Characterization of the Thermally Tolerant Pectin Methylesterase Purified from Citrus sinensis Fruit and Its Gene Sequence. Journal of Agriculture and Food Chemistry 61(51): 12711-12719 3. Yang Kim, Martin A. Williams, Ashley L. Galant, Gary A. Luzio, Brett J. Savary, Prasanna Vasu, Randall Cameron. 2013. Nanostructural Modification of a Model Homogalacturonan with a Novel Pectin Methylesterase: Effects of pH on Nanostructure, Enzyme Mode of Action and Substrate Functionality. Food Hydrocolloids 33: 132-141 4. Prasanna Vasu, Brett J. Savary, and Randall G. Cameron. 2012. Purification and characterization of a papaya (Carica papaya L.) pectin methylesterase isolated from a commercial papain preparation. Food Chemistry 133: 366-372 5. Prasanna Vasu, Stefan Bauer and Brett J. Savary. 2012. Cloning and expression of hemicellulases from Aspergillus nidulans in Pichia pastoris. Methods in Molecular Biology. Recombinant Gene Expression: Reviews and Protocols, 3rd ed.; Lorence, A., Ed.; Humana Press/Springer: New York. 824: 393 - 416. 6. Brett J. Savary and Prasanna Vasu. 2012. Routine identity confirmation of recombinant proteins by MALDI-TOF mass spectrometry. Methods in Molecular Biology. Recombinant Gene Expression: Reviews and Protocols, 3rd ed.; Lorence, A., Ed.; Humana Press/Springer: New York. 824: 37 - 50 7. Randall G. Cameron, Gary Luzio, Prasanna Vasu, Brett J. Savary and Martin Williams. 2011. Enzymatic Modification of a Model Homogalacturonan with the Thermally Tolerant Pectin Methylesterase from Citrus: I. Nanostructural Characterization, Enzyme Mode of Action and Effect of pH. Journal of Agriculture and Food Chemistry 59 (6): 2717–2724. 8. Brett J. Savary, Prasanna Vasu, Alberto Nuñez, and Randall G. Cameron. 2010. Identification of thermolabile pectin methylesterases from sweet orange fruit by Peptide Mass Fingerprinting. Journal of Agriculture and Food Chemistry 58 (23): 12462-12468. 9. Prasanna, V., T.N. Prabha and R.N. Tharanathan, 2007. Fruit ripening phenomena-An overview. Critical Reviews in Food Science and Nutrition 47(1): 1-19. 10. Bauer, Stefan, Prasanna Vasu, Staffan Persson, Andrew J. Mort and Chris R. Somerville, 2006. Development and application of a suite of polysaccharide degrading enzymes for analyzing plant cell walls. Proceedings of the National Academy of Sciences (PNAS USA) 103 (30): 11417-11422.